SAMD21G18A Controller,s Bootloader Delete after program with Arduino IDE

first i programmed the bootloader from atmel studio 7 then after that i tried to program from Arduino ide, packages are proper install but when i program and upload successfully code then controller doesn,t detect. currently using feather M0 board. and also tried restore bootloader with adafruit solution learn.adafruit.com/prope.... but doesn,t happen. Currently using Replica  of Adafruit Feather MO made by myself.

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